The Development of Fluorescence Intensity Standards
نویسندگان
چکیده
The use of fluorescence as an analytical technique has been growing over the last 20 years. A major factor in inhibiting more rapid growth has been the inability to make comparable fluorescence intensity measurements across laboratories. NIST recognizes the need to develop and provide primary fluorescence intensity standard (FIS) reference materials to the scientific and technical communities involved in these assays. The critical component of the effort will be the cooperation between the Federal laboratories, the manufacturers, and the technical personnel who will use the fluorescence intensity standards. We realize that the development and use of FIS will have to overcome many difficulties. However, as we outline in this article, the development of FIS is feasible.
منابع مشابه
Pharmacognostic and Elemental Analysis of the Leaves of Tapinanthus globifer (A. Rich). Tiegh
Background and objectives: Tapinanthus globifer is a semi-parasite plant that mostly grows on the branches of the host tree species of the genera Vitellaria, Kola, Citrus, Combretum, Acacia, and Terminalia. The leaf is known for its use in traditional medicine to treat inflammations, malaria, headaches, bacterial infections, ulcers and diabet...
متن کاملDevelopment of a Simple and Sensitive Terbium Sensitized Fluorescence Method for Determination of Epinephrine and Norepinephrine in Serum
A simple, rapid, selective and highly sensitive fluorimetric method for determination of two catecholamines, i.e. norepinephrine (NE) and epinephrine (EP), in serum samples was developed. The method is based on the fluorescence sensitization of terbium (Tb3+) by complexation with both catecholamines in the presence of lanthanum (La3+), as a co-cation, and in a Na...
متن کاملFluorescent standards for photodynamic therapy
Photodynamic therapy is an evolving technique for treatment of various oncological diseases. This method employs photosensitizers – species that lead to death of tumor cells after the photoactivation. For further development and novel applications of photodynamic therapy new photosensitizers are required. After synthesis of a new photosensitizer it is important to know its concentration in diff...
متن کاملDevelopment of an Alu-PCR Amplified YAC Probe Suitable for Enumeration of Chromosome 13 on Uncultured Lymphocytes and Amniocytes by Fluorescence in situ Hybridization
The main objective of the present study was to develop an efficient and reliable probe to be routinely used for detection of chromosome 13 copy numbers by interphase FISH. To achieve this, a Yeast Artificial Chromosome (YAC) containing sequences specific for human 13q12 (744D11), was cultured and the whole yeast genomic DNA was extracted. The human insert within the isolated DNA was amplified b...
متن کاملThe fluorescence Quenching Study of Quinine in Presence of Some Anions
The quenching of quinine fluorescence intensity in the presence of some anions in aqueous solution at ambient temperature has been investigated. The quenching is found to be collisional or dynamical in nature. This study reveals the order of two groups of quencher: NaI > NaBr > NaCl > NaF and K2Cr2O7 > KMnO4 >Na2SO4 > NaClO3. Increasing anion size in the both groups leads to an increase in the...
متن کامل